Tyrosine 3-hydroxylase in rat brain and adrenal medulla: hybridization histochemistry and immunohistochemistry combined with retrograde tracing.
نویسندگان
چکیده
منابع مشابه
Cycloheximide increases proenkephalin and tyrosine hydroxylase gene expression in rat adrenal medulla.
The effect of cycloheximide (CHX; 5 mg/kg) on proenkephalin (proENK) and tyrosine hydroxylase (TH) mRNA expression in rat central and peripheral nervous systems was studied. CHX increased proENK and TH mRNA levels in the adrenal gland, but not in hippocampus, striatum, midbrain, brainstem, pituitary, and hypothalamus. The pretreatment with actinomycin D (0.5 mg/kg) significantly decreased CHX-i...
متن کاملStudies on tyrosine hydroxylase from bovine adrenal medulla.
Approximately 90% of the total tyrosine hydroxylase activity of bovine adrenal medulla homogenates was localized in the particulate fraction of the homogenate. The particulate enzyme was solubilized by incubation with trypsin, and the solubilized enzyme was partially purified. This enzyme was found to behave like the tyrosine hydroxylase which had been purified from the supernatant fraction by ...
متن کاملRegulation of tyrosine hydroxylase activity by muscarinic agonists in rat adrenal medulla.
Regulation of tyrosine hydroxylase (TH) enzymatic activity in vivo by muscarinic receptor agonists in rat adrenal medulla was characterized in this study. Bethanechol and carbachol produce dose-dependent increases in rat adrenal TH activity. These increases are maximal (approximately 3-fold) using 10 mg/kg bethanechol or 0. 5 mg/kg carbachol and are totally inhibited by prior administration of ...
متن کاملThe Effects of Phenoxybenzamine and Cold Stress On Tyrosine Hydroxylase Enzyme Activity and Expression of Tyrosine Hydroxylase Gene in the Rat Adrenal Medulla
The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydroxylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intraperitoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by...
متن کاملTyrosine hydroxylase activity and catecholamine biosynthesis in the adrenal medulla of rats during stress.
Chronic hypotension and hypoglycemia are known to increase the capacity for catecholamine biosynthesis in the rat adrenal medulla by increasing the maximal velocity of the rate-limiting enzyme, tyrosine hydroxylase (TH). The present report indicates that the gradual increase in maximal TH activity is preceded by a more rapid increase in the affinity of TH for its pterin cofactor. These short-te...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.16.6208